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KMID : 1177720100040020023
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Journal of Alternatives to Animal Experiments
2010 Volume.4 No. 2 p.23 ~ p.28
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The New Evaluation Method for Volatile Material and Formulation Containing Volatile Materials Using Modified HET-CAM Assay
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Cho Sun-A
Kim Jong-Hyub
Lee Tae-Ryong
Shin Kye-Ho
Kim Han-Kon
An Su-Sun
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Abstract
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Although ammonia can cause eye irritation such as lacrimation, periobital edema and conjunctivitis at high concentration, it has some merits in hair dying function and has been commonly used in hair dye product. However, there are no proper alternative methods for evaluation of the eye irritation potential of volatile material such as ammonia. We have established a new method for determination of eye irritation potential of volatile materials and formulations containing volatile materials by modification of HET-CAM (Hen’s egg test- chorioallantoic membrane) assay. While original HET-CAM assay cannot determine the eye irritation potentials of hair dyes that contain ammonia, modified method can determine the eye irritation potentials of hair dyes that contain ammonia. These results suggest that the modified HET-CAM assay is useful to evaluate the eye irritation potential of volatile materials.
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KEYWORD
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HET-CAM, Eye Irritation, Alternative Method, Volatile Material, Ammonia, Hair Dye
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